Protein sensors
Light induced dimerization (LID)
Integrated phage display, yeast and mammalian two-hybrid screening of a combinatorial nanobody library against a truncated bacterial phytochrome sensory module comprising a mammalian endogenous chromophore, biliverdin, to create red light-switchable protein dimerization systems:
Creating Red Light-Switchable Protein Dimerization Systems as Genetically Encoded Actuators with High Specificity. Zhimin Huang*, Zengpeng Li*, Xiao Zhang*, Runze Dong, Shoukai Kang, Li Sun, Xiaonan Fu, David Vaisar, Kurumi Watanabe, Liangcai Gu#, ACS Synthetic Biology, 2020, 9, 3322
Similar combinatorial approaches were proposed to design other photoswitchable heterodimerization systems, opto-binders, and photocaged ligand-controlled CID systems:
Combinatorial Approaches for Efficient Design of Photoswitchable Protein-Protein Interactions as In Vivo Actuators. Xiao Zhang, Yuxin Pan, Shoukai Kang, Liangcai Gu#, Frontiers in Bioengineering and Biotechnology, 2022, 10 (doi: 10.3389/fbioe.2022.844405)